can someone recommend a biovendor where I can purchase active human FGF21 protein?
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
I am trying to compensate my flow cytometry data. I have human tumor tissue, dissociated and stained with live/dead fixable::APC, EpCAM::FITC, CD45::PE. I also stained and measured single stain...
07 July 2024 5,614 5 View
Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!
26 June 2024 9,093 4 View
Hi! I have to test a drug on the LSC fraction of THP1, but I'm not finding any specific media to select and culture them. Do you know anyone? Thank you in advance
15 May 2024 1,464 3 View
Hello, I am performing esterification reactions between fatty acids and alcohols, in presence of methanesulfonic acid and H3PO2 as catalysts. At the end of the reaction, to reach the acid index I...
22 April 2024 2,063 5 View
I need to analyze the residual methanol from methyl esters. I am wondering if there is someone who has this method DIN EN 14110 and is willing to share it with me? Thank you in advance, Elena
22 April 2024 387 0 View
Recently my pepsin column got some blockage, supposedly because of the precipitation of aggregated protein. Multiple washes with 0.1% FA or pepsin wash (1.5M GuHCl + AcN + FA 95.2/4/0.8), as well...
20 April 2024 3,135 5 View
I am facing an issue with how chromatograms are recorded. From time to time, on our chromatograms the peak assigned to heptane is integrated as well and this gives us wrong results. Sometimes the...
07 April 2024 1,726 4 View
I need to perform non-parametric post hoc multiple comparisons. I found two interesting macros that implemented methodology about post hoc test for Kruskal Wallis test, but p-value was never...
07 April 2024 288 3 View
Dear colleagues, I am looking for advice on the validation of a standard questionnaire that I intend to translate. The original version contains 40 items. Could you please tell me what sample...
26 March 2024 1,660 11 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...
02 August 2024 6,773 12 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...
28 July 2024 6,515 1 View
Perfect democracy thinking assumes no chaos so no need for independent rule of law system and liberal democracies assume the possibility of normal democratic chaos that can be sorted out by an...
28 July 2024 473 1 View
Please send me updates regarding the above-mentioned areas in the question mark?
28 July 2024 5,197 3 View
Probiotics related.
22 July 2024 1,707 2 View
What is human-computer interaction (HCI)?
22 July 2024 10,056 2 View
Hello researchers, I have sequenced the A549 cell line using RNA sequencing approach and then I have it against the human genome reference sequence. Would I expect a 100% mapping percent? I got...
20 July 2024 9,705 0 View