I was built using two distinct phylogenetic trees created by MEGA X. The first tree was created using the ITS sequences of 24 different species of mushrooms, and the second tree was created using nuc-LSU sequences, which contain the same number of sequences (taxon), using the calculus models of Tamura-Nei and Maximum likehood
Can someone help me to discuss the result (tree)?
Phylogeny is made up of branches and nodes. The closer the species are to the genus, the more conserved they are, and the further away they are from the clade, the more mutations, conservations, deletions or insertions from the origin have been suffered. Nodes are the common ancestors of the genus.
What was your original goal in generating the two phylogenetic trees? What model would you like to use to interpret your results?
Sincerely.
If your answer has not been answered I would be happy to look at your results.
yes of course, those are the two tree realized by MEGA 11 under Maximum Likehood algorithm and Tamura and Nei model.
this is my discussion:
As a preliminary inquiry, two trees are demonstrated in the study to determine the Algerian mushroom C. cylindracea grouping. The results revealed the significant resemblance between the Algerian strain MEST42 and those from European countries.
Most scholars have adopted the construction of the phylogenetic tree of fungal multigene fragments, which is more conducive to the correct definition of difficult species or similar species (Zhou et al, 2022). The reason of the use two markers ITS and nuc-LSU in this study, to understand the phylogenetic relationship of the Algerian strain with the world strain of A. aegerita.
It was noted that a number of factors including climate, geography, and environmental changes, can have an impact on the preservation of the genetic materials of species from the genus Agrocybe(Cyclocybe), which are susceptible to changes and can cause numerous morphological, physiological, and biochemical changes (Zhou et al, 2022; Frings et al, 2020).
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The clades are on the right, and the left is the primary origin of the genus. The numbers are the distances in relation to the origin; the further away they are, the more distant they are, the more likely there was deletion, mutation, or evolution. In mega11, the color in the yellow case, or orange, is the color of interest. It groups the branches by species. In this case, the numbers are measures of genetic distance between the DNA sequences of the samples, that is, they are evolutionary changes that give us a genetic similarity. Please, in mega11, compare the sample of your interest with the other closest species and you will see if there are samples preserved or not by asterisk. At your service.
If I understand your question correctly, you would like some advice on how to build up your discussion part for the phylogenetic trees attached. You seem to have written a few sentences containing different ideas, but haven't discussed each of those thoughts. Try making a plan for how you want your discussion to look like first, and then write one-two sentences describing what you want to talk about in that particular part of the discussion. Next, build on that. Look for papers that support your thoughts and compare your results, discuss their implication based on those papers. Keep your writing organized, concise and precise, and make sure every paragraph is actually a different thought (topic) for discussion.
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