Extracting blood from rats that age has been proven to be difficult for many of our lab members.
There are so many methods are available to quantify oxytocin and vasopressin in 21 days old rats. You can develop your own method, first you should extract out peptide from faces and urine, then use antibody based assay for quantification of both hormones.
J Gerontol B Psychol Sci Soc Sci. 2014 Nov;69 Suppl 2:S229-37. doi: 10.1093/geronb/gbu104.
Social peptides: measuring urinary oxytocin and vasopressin in a home field study of older adults at risk for dehydration.
Reyes TL1, Galinsky AM2, Hoffmann JN3, You HM3, Ziegler TE4, McClintock MK5.
Vasopressin ELISA kit - Enzo Life Sciences
www.enzolifesciences.com/ADI-900-017/arg8-vasopressin-elisa-kit/
Cited sample types include plasma, serum and urine
Hi Raura, recommend you read the paper by Gareth Leng and Nancy Sabatier 2016 J Neuroendocrinology. its not quite as straightforward as isolation and then analysis with antibodies - not saying you can not do it, but be aware there are several controls required and careful selection of which antibody to use. not sure commercial assays are a good way to start. Professor Hal Gainer has fantastic antibodies for oxtocin and vasopressin. you might try contacting him. or contact Rainer Landgraf whose lab runs a very safe dependable assay. hope this of help
- Badges
- Science topic
- Similar topics
- Medicine
- Disease
- Disease Models
- Animal Models
- Rodent Models