You may use 3% SCDM (100 ml) and inoculate atleast 1 ml of your sample to set of bottles (8 Nos for bacterial detection, 8 for fungal detection and incubate in appropriate temperature).
Refer EU / US pharmacopeia.
@Sekar Thangaraj Thanks for your attention and reply. But it has oily identity and couldn't be solved in SCDM as you know.
Hi Golnaz,
The filtration techniques (0.22um) is also not helpful for the oily sample.
Still I suggest that you may try with 100 ml of sterile 3% SCDM and inoculate 1~2 ml of your sample and mix well before placing for incubation.
The suspected contaminants (if present in your sample) in your oily samples need to diffuse in to the media and the growth can be detected.
You may spick your oily sample with very little quantity of known organisms and check the detection ability of SCDM for your sample.
The antiform in the bacterial culture media ( for vaccine manufacturing) is high density than the oil and we are using SCDM to check the sterility of it.
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