I need to detect the protein c-Fos in rat brain by immunohistochemical methods. Could anyone suggest a sensitive protocol?

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Rosie Morland

Are you planning on using mounted or free-floating sections? I use a santa-cruz ab at 1:20,000 with DAB visualisation with free-floating sections and more than happy to attach a protocol if it'd be of use

Xavier Matias-Guiu

It is sometimes useful to use as controls, cell lines in whick the target gene has been silenced by siRNA. This is a very good control to check specificity. We performed that several times. Please check the article: Mod Pathol. 2005 May;18(5):719-27

Fatiha Chigr

Please see this technical article:

c-Fos expression as endogenous marker of lumbosacral spinal neuron activity in response to vaginocervical-stimulation.

Ghanima A, Bennis M, Rampin O.

Brain Res Brain Res Protoc. 2002 Feb;9(1):1-8.