How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How can AI be used for enabling Design for Sustainability?

Design for Sustainability (DfS) is a wicked and complex problem. It needs the designer to combine specialized knowledge from several disciplines with systemic thinking. The designer also needs to...

05 February 2021 2,998 9 View

How can I improve RNA extration from human neutrophils?

I tried several times extrating RNA from human neutrophils and the amount of RNA at the end of the precedure was minimal. I also tried using colunns from QIAGEN. Never had any problem with tissue...

26 January 2021 8,881 4 View

How to assess monoamine oxidase levels ?

hello everyone i have a research about monoamine oxidase enzyme and i saw a lot of detection method of it including monoamine oxidase activity assay kit but i don't know what is the easiest and...

18 January 2021 3,474 2 View

Is concrete block bricks eco-friendly?

Concrete block bricks companies are marketing that their products are eco-friendly, as they are not emitting carbon dioxide and not using soil. At that viewpoint, they are right. But,in their...

04 January 2021 5,641 8 View

What possible journals do you suggest for an exploratory study on learning sustainabilty competence?

Hello! We are conducting a study that practically implemented a combination of different learning approaches to foster systems thinking competence in the context of sustainability and in a...

17 December 2020 6,554 2 View

ELISA detection cytokine in human serum (common dilution fold)?

Hi everyone, I'm gonna try to detect the IL-10, IFN-y, TGF-beta1 level in the human serum by ELISA. I bought the ELISA set from the BD contain Capture Ab, Detection Ab, Enzyme reagent, Standards....

09 December 2020 3,369 5 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View

How to compare two fMRI sessions using SPM12 (inter-sessions differences) ?

I'm performing an fMRI analysis for my master thesis using SPM and I've never worked with fMRI data before. I've succesuffy perform first level analysis but came to an obstacle. Study design...

05 December 2020 6,956 2 View

Does anyone know the neutrophil marker for qPCR in human gut (duodenum, ileum, colon) biopsies?

Hello everyone. I have collected intestinal biopsies from stem cell transplanted patients in RNAlater and wish to quantify neutrophils only via qPCR. As we know that the mucosa of gut is filled...

25 November 2020 2,680 3 View

Has anyone observed delamination of pyrolysed SU-8 on SiO2 coated Silica substrates when the pyrolysis is performed outside cleanroom?

We have a quartz tube furnace (diameter 80 mm) outside the cleanroom and when tried to pyrolyse the SU-8 on SiO2 coated Si substrates at 900 C in nitrogen atmosphere, the electrodes looks fine....

08 November 2020 4,357 2 View