How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

Why the CHi^2 is close to zero with a bad fitting but the Rp and Rwp are still high?

I am refining my XRD data using in FullProf program. The intensity of the modulated X-ray peaks is almost flat. I tried to adjust the peak intensities but unsuccessful. What is the reason? I guess...

23 February 2021 2,304 4 View

Rietveld refinement of cement paste?

I am a beginner in rietveld refinement, my samples contain numerous overlapped peaks and amorphous phases. I have few doubts related to rietveld refinement of cement paste: 1. My quantifications...

11 January 2021 8,873 6 View

When i accept card in "HighScore plus app" should i attention to cards quality (in Reitveld analysis)?

Hi I wanna use Reitveld analysis in my project but i have big question that is: Should i see quality of each card (or Not important !!?), or it is enough to check the score and scale factor of...

04 January 2021 7,750 1 View

Which analysis method in XRD analysis is the most accurate way to measure crystallite size & lattice starin? Scherrer, Williamson-Hall or Rietveld?

Here are three different ways to calculate crystallite size and lattice strain of a sample, based on XRD peaks: "Scherrer" calculation, "Williamson-Hall" or "Rietveld". Each calculation is proper...

04 January 2021 7,836 4 View

How could I fix the "negative intensities in scan data points" error in X'pert when applying Rietveld analysis?

I have faced a problem in using Xpert in Rietveld Analysis, I got the abovementioned message when trying to run Rietveld analysis. The cause of the problem is probably due to that the spectrum has...

28 November 2020 4,272 4 View

Can someone advise me to do Rietveld Refinement of the material whose Crystallographic Information Framework (CIF) file is not available?

10 October 2020 7,411 9 View

How to fix the refinement on Fullprof?

I have an XRD pattern of malachite phase in a range of 5-50 degree in 2 theta. After that, I use MID-JADE software to refine the pattern and it shows a good refinement with low R-value and GOF...

03 October 2020 3,613 8 View

What should be the Bragg R-factor value to stop at while performing the Rietveld Refinement?

How does the Bragg-R factor value effects the chi-square value?

22 September 2020 5,784 4 View

How is it possible to simulate or model particle morphology using X-ray powder diffraction?

Hello everyone! I suppose the XRD of sample LaPO4 has some anisotropic line broadening along [h00]. How could I model the shape of particle using WPPF method or something else? Diffraction...

21 September 2020 1,823 3 View