Dear Mohamedahmed,

Maybe the bradykinin chapter in the following "Laboratory standard operating procedures" will help you on the subject: CKD Biomarkers Consortium Steering Committee. Laboratory standard operating procedures. 2013. http://www.ckdbiomarkersconsortium.org/laboratory_SOPs.pdf

And maybe the “Material and Methods” section of the following papers can also help you:

Murphey LJ, Hachey DL, Vaughan DE, Brown NJ, Morrow JD. Quantification of BK1-5, the stable bradykinin plasma metabolite in humans, by a highly accurate liquid-chromatographic tandem mass spectrometric assay. Anal Biochem 2001;292(1):87-93. https://eurekamag.com/pdf/011/011231980.pdf

Simões HG, Asano RY, Sales MM, Browne RA, Arsa G, Motta-Santos D, et al. Type 2 diabetes elicits lower nitric oxide, bradykinin concentration and kallikrein activity together with higher DesArg(9)-BK and reduced post-exercise hypotension compared to non-diabetic condition. PLoS One 2013;8(11):e80348. https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0080348&type=printable

Asano RY, Browne RAV, Sales MM, Arsa G, Moraes JFVN, Coelho-Júnior HJ, et al. Bradykinin, insulin, and glycemia responses to exercise performed above and below lactate threshold in individuals with type 2 diabetes. Braz J Med Biol Res 2017;50(11):e6400. http://www.scielo.br/pdf/bjmbr/v50n11/1414-431X-bjmbr-1414-431X20176400.pdf

Seip KF, Bjerknes KC, Johansen HT, Nielsen EW, Landrø L, Reubsaet L. Bradykinin analysis revived--a validated method for determination of its stable metabolite in whole blood by LC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci 2014;947-948:139-44. https://www.researchgate.net/publication/259724741_Bradykinin_analysis_revived_-_A_validated_method_for_determination_of_its_stable_metabolite_in_whole_blood_by_LC-MSMS

Best regards from Germany,

Martin

Thank you

Hi Eltohami,, very good question. Diabetes is an important cause of cardiovascular and renal disease. Alterations of bradykinin forming component is seen in diabetic animals. High urinary and issue kalikrein is seen in diabetic patient.. May have some role in pathogenesis of renal and cardiac complication.. Following article might help you.. Thanks