i am working on RNAi and going to make transgenic tobacco plants which will show resistance against sucking insects. At the initial stage i need pcambia based RNAi vector which have both 35s promoter and kanamycin resistance genes.
We used the vector pK7GWIWG2D, (Gateway technolgy, from Invitrogen) that was really practice and fast to contruct. It is a little expensive but useful. It has 35S promoter and Kan resistance to plants selection. It is a vector to generate silencing of some gene/s. Please find attached our paper where we use it.