Dear Mohammed

Like it is explained at INVAM´s web page (https://invam.wvu.edu/methods/cultures/field-transplants), for some research projects, special consideration is given to maintaining the same association between fungi and their host in the greenhouse as in the field. One way to do this is to sample intact plants from a suitable field site and transplant each into pots with sterilized soil. These plants would be grown for at least 3-4 months before collecting a sample to assess sporulation.

Some years ago, we also sampled the interested plant from field soils, cut the roots, put them in a pot with sterilized soil, and we collocated pre-germinated Sorghum seedlings in order that these roots functioned like a mycorrhizal inoculum for the new plants. After 4 months the soil need to be checked to observe the sporulation. Best regards, Eduardo

Examination of samples from fungus roots under a microscope to confirm that the roots are infected with mycorrhea after staining with Acid fuchsin and according to the method (Phillips and Hayman 1970,). Mushrooms are diagnosed depending on the taxonomic qualities cited (Blaszkowski et al., 2009)

Abdulnabi Abdulameer Matrood your article is very useful for this question