MSD requires 8 standards that are serially diluted down in order to produce a standard curve. In the final row of my MSD Assay, I accidentally pipetted DMSO (same volume) into the last 4 wells of the serial dilution, when it should have been into the 4 wells of diluent (in the same row). The DMSO concentration/ECL signal in the diluent would have helped normalize my data for analysis, but now I do not have a way to normalize due to my error. Plus, my standard curve now has to be only 4 points instead of 8 since the last 4 dilutions now have DMSO in them.

I didn't mess up any of the compounds in the above 7 rows, but now I cannot think of a way to be able to normalize my data either via ECL signal or DMSO concentration. Any thoughts?

My best thought, though I don't know how feasible:

The final well of the serial dilution (which received an accidental dose of DMSO) contained 300 microliters diluent.

The wells that were supposed to receive the DMSO contained 50 microliters of diluent.

Since the volume of DMSO would have been the same in both wells, could I perhaps scale up my ECL signal by a factor of 6 for the 300 uL well so I can figure out what the normalized signal should have been in the 50 uL well? For example, if I got an ECL reading of 30,000 RLU in the 300 uL well, could I infer that the reading would have been ~180,000 RLU in the 50 uL well since the DMSO would theoretically be 6x more concentrated?