I have prepared a fmoc amino acid based peptide having both the SH groups are protected with triphenylmethyl (i.e, trityl) group. Actually I want to do mono deprotection. I have a little compound So, Could you please share your advice/experience? Thanks in advance.
Totally agree with Stjepan Krešimir, use different protection groups for the thiol so you can remove just one of them orthogonally.
Stjepan Krešimir Kračun
Thank you for your response. The peptide which I am working that is very difficult to introduce bi-functional protection groups. Using both Trt unit I am able to get decent yields. I have other limitations also. Thank you :)
Iris Bermejo Thank you :) My base materials are bis (2-chloroethyl) amine and Trt-SH. In this way I am preparing the peptides Trt-S-------Peptids----S-Trt. If I can able to make one free SH is much easy to go next step. I want to try 1 equivalent TFA ??and other Need help. Thanks
Stjepan Krešimir Kračun
Thank you for the detailed information. By using the resin might solve my problem otherwise i will alter the synthetic plan. I will also try the NCL. Thanks you so much :) :)Best
Prathap
Perhaps some inspiration here with the orthogonal protection scheme for thiols:
Nature Chemistry volume 1, pages635–641(2009)
or perhaps some variants of photo cleavable protection groups, here some self-promotion,and not directly for thiols, but several examples should be applicable:
Article ChemInform Abstract: A Photolabile Protection Strategy for T...
Article Release of Terminal Alkynes via Tandem Photodeprotection-Dec...
Kasper Moth-Poulsen Thank you :) Photodeprotection strategy is really cool !
I can think(try) in this direction to introduce some of photolabile protecting groups.
Thank you for nice idea:) :)
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