Desing a New reverse primer for coi with a his tag and the stop codon, it would be a 50pb primer. 

Thank you Jahaziel. We have that in mind. Was looking for suggestions on whether should we go ahead with the same constructs and give it a shot?

Hi Hiral,

I would just go ahead and give it a try - initial expression screens will only take a couple of days...If you can not see soluble expression, I would recommend to clone the GOI without any extra codons (not even a Histag to see if the native protein can be solubly expressed in E. coli. Good Luck!