why do you want to insert such genes which will destroy plant cell? What is the need of inserting such genes into plants which will destroy the plant? When we insert a gene, it should have some good impacts on the plants, if the gene itself is harmful for the plants then what is the importance of such genes. But yes, if your gene is meant to provide biotic-stress resistance, then some different strategies can be taken. But it is always best to take such genes which is never harmful for the plant itself.

If i am correct, your question is, "how to obtain (grow) a transgenic plant with a gene whose product is toxic to the plant".

Under such condition you have to use a Inducible Promoter Systems. Your Gene of interest will be expressed only when it is induced but under normal uninduced state the plant will be normal. You can refer to the article below.

https://doi.org/10.1046/j.1365-313x.2000.00868.x

Hope i understand your question correctly .

You could perform a transient transformation of plant tissues using either costitutive promoter or an inducibile one or by floral dipping using an inducibile promoter in order to let the plant develope before expressing the gene.

Either way it will cause damage according to the activity of the protein expressed on the different tissues cells.

It is unlikely that the gene would be silenced using a common promoter belonging to the afore-mentioned categories.

I can't imagine why someone would produce such plant.

A number of research groups are interested in transgene containment strategies, and controlled expression of negative selection markers are one approach to accomplishing this goal. Bacterial RNAse (Barnase) is an example of a gene that has been used to eliminate plant cells in which is expressed, so you could perform a literature search for this. The response from Vivek nicely points out that an inducible promoter is necessary to get this strategy to work. Inducible promoters include those which need some sort of chemical to be added (ethanol or estradiol), or could be a temperature sensitive promoter (there are reports of heat inducible promoters), but we could also include in the collection native promoters that are tissue or cell specific, or are only driving gene expression at specific points in plant development, such as a pollen specific promoter. Bottom line, there are many options from which to choose, and all approaches required thoughtful planning and thorough experimentation to successfully implement.

"What happens if after the introduction of DNA into plants ,the plasmid DNA is coding for a protein that will destroy plant cells" ----The plants may die.

Former answers are clever... In some other fields such as therapeutic proteins within plant cells *suspension cultures*, some recombinant proteins are targeted to vacuoles or the RE so that proper glycosylation of mAb be performed. You could take this approach to target your killing protein to a vacuole instead of the cytoplasm, of course this would be so much easier in suspension cultures than transgenic plants. You could also consider changing your platform of production... Let us know how you resolved !