In the green synthesis process, we make the aqueous solution of precursors and put it on the hot plate and stir it. Then to this precursor solution, we add the aqueous extract of the plant where the constituents of plants work as reducing as well as capping agent. Finally, we add some base solution to increase the pH. My doubts are:
1. While adding the plant extract in the precursor solution, what is the role of heating there?
2. If we use purified constituent of the plant, which is working as a reducing agent it could be also a green/biosynthesis of nanoparticles?
Increasing temperature can facilitate the reduction of your initial salt, and can increase the reaction rate that subsequently impacts on the shape (spherical or polyhedral) and size of nanoparticles.
Of course, other circumstances such as kind of compounds, pH, the order of mixing, speed of stirring or etc... can influence on their shape, size, and distribution.
Your synthesizing is green on condition that your purified constituent of the plant has low cytotoxicity. You can investigate this issue by MTT assay. For example, if you synthesis nanoparticles via pure glucose, that will be a green method.
I agree with Uzma Bibi. In addition, the heat is to allow as much quantity as possible of the plants' constituents to go into the water as the solvent.
Secondly, as long as the isolated constituents are soluble in water and you use them as such, the process is still green. The idea of green is to avoid toxic and harsh chemicals as much as possible.
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