I am Kodai Fukada, a member of the Chemistry Department at UNIVERSITY OF TOYAMA FACULTY OF HUMAN DEVELOPMENT ATTACHED SCHOOL. I can secure beetle larvae, but I don't have HPLC, so I can't purify them. Please answer me!

Beetle defensins are antimicrobial peptides found in the body of beetle larvae. It kills germs that invade the body of the beetle larvae in decomposing soil with a lot of bacteria, and prevents infection. Beetle defensins were discovered in 1996 by Miyanoshita et al. of the then National Institute of Silkworm and Insect Science (now National Institute of Agrobiological Resources). The number of amino acid residues is 43, and the sequence is as follows vtcdllsfea kgfaanhslc aahclaigrr ggscergvci cre

Beetle defensins are antimicrobial peptides, and many such antimicrobial peptides and proteins have been found in invertebrates. In particular, many insect-derived peptides exhibit antibacterial activity by disrupting bacterial cell membranes, and are being studied as antibiotics that are resistant to drug-resistant bacteria such as methicillin-resistant Staphylococcus aureus. Beetle defensins are also thought to have strong basicity, which may be effective in puncturing the phospholipid membranes of drug-resistant bacteria and causing them to lyse. In order to be used in clinical medicine for humans, the number of amino acid residues that make up beetle defensins needs to be modified. This is because if the full-length beetle defensins are administered into the human body, the antigen-antibody reaction will eliminate the beetle defensins from the body. To avoid this, attempts have been made to reduce the number of amino acid residues to about 10 while maintaining the antibacterial activity. In addition, research is underway to use beetle defensins clinically as anticancer agents. Some of the amino acid modified peptides derived from beetle defensins have been reported to be selectively cytotoxic to cancer cells only [5]. In general, cancer treatment involves chemotherapy with anti-cancer drugs and radiotherapy, but these methods also destroy normal tissues such as white blood cells, resulting in so-called side effects. Depending on the specificity of the cytotoxicity shown by the beetle defensin, a new drug with fewer side effects may be born.