Dear Ali,

Would you please tell me the lab from where you sent your bacteria for FAME analysis?

Thanks.

On the basis of FAME analysis also you can tell about the identification of bacteria. The labs doing FAME analysis have the library of characterization on the basis of fatty acid profile. The companies routinely doing FAME analysis have these library and they provide you about the characterization also. The Indian companies routinely do this on mass scale. I have got many bacteria characterized with FAME analysis. Basically FAME is a supportive data in addition to 16sRNA and biochemical Characterization. With addition along with 16S you can publish high impacts while only with FAME you will be able to publish in less impacts. The fatty acid profile provides you about the ancestry of bacteria. You can search there are certain fatty acid which are found only in extremophiles which are considered more ancestors to normal bacteria. So if you want to know on your own you have to little bit of search, alternatively you can ask your lab to match with library so that you can get the possible identification

@Kunal- i sent my bacterial culture to IMTECH,Chandigarh for FAME analysis. Now they are denying for identification of bacteria and provided only Fatty acid profile.

Ali Asger, 

They cannot name the bacteria species if they have no simiar FAME profiles in own database. Sometimes close species and even strains have very different FAME profiles.

Dear Ali,

   Please carry out 16S rRNA gene sequencing by sending either genomic DNA or strain by outsourcing (many companies are available for example Amnion biotech, axcelaris etc). one you have the results, go for its near relative search using Eztaxon server.Once you get idea of near relatives, retrieve papers describing the respective novel taxa. Recently a new the threshold value for novel sp. has been proposed which is 98.5-99% (Kim et al., 2014; IJSEMpaper based on ANI-DDH-16S rDNA study), earlier it was 97% (stackebrandt & Goeble, 1994).

Now find out the FAME profiles of near relatives and compare the same with that of yours. Remember, these should be carried out after growing bacteria under identical condition (usually condition for growth for TSBA method is growth on TSA at 28 degree Celsius).

But even if you have both these data you can not prove if your strain is novel. for that you have to go for detail polyphasic analyses. if your strain is Gram positive one you must do cell wall and menaquinone analyses. for actinobacterialisolates chemotaxonomic analyses are much more regorous.

You can contact scientists (Dr.Kamlesh Jangid/Dr. Om Prakash Sharma) from MCC, NCCS pune. It is also an IDA & they have identification services. They have sufficient infrastructure, equipment to solve your problem. Please visit to MCC web site.

I hope it helps.

with best wishes

Pradipta

You can contact Royal Life sciences Secunderabad, Telangana www..royalgroupinfo.com for Microbial Identification .they do analysis of PLFA, FAME with MIDI and 16S rRNA sequencing

Using FAME-MIDI analysis they assign to genera. But related bacteria can have different profile and the results depends on cultivation conditions and isolation source.

So, You will need 16 S sequence and other analysis for your identification.

Hope this helps

Where can I send these bacteria for chemotaxonomic characterization (FAME analysis and polar lipid)?

Roshibina Khumballambam you can contact IMTECH Chandigarh or any SAIF laboratory.