How to use a Western Blotting specific antibody for IHC (for PFA fixed & paraffin Embedded tissue)?

I have an antibody in my lab specefic for western blotting only, but I need to detect same antigen in a PFA fixed tissue. I tried to retrieve epitopes using Citrate buffer, HCL treatment &...

09 October 2014 6,765 8 View

Can we use DMSO as a safe solvent to dissolve curcumin for oral administration?

Is DMSO safe for mice since I need to administer curcumin orally to them by dissolving it in DMSO.

04 May 2014 7,099 34 View

how to prepare Ca and Mg free PBS?

how to prepare Ca and Mg free PBS for cell isolation

06 July 2013 5,184 0 View

What can be the reasons for lesser precipitate formation in RNA in situ hybridisation in a PFA fixed & paraffin embedded adult murine tissue?

I am getting very little precipitate in RNA in situ hybridisation for my paraffin embedded adult murine tissue. How can I enhance the purple precipitate in the tissue?

05 June 2013 2,983 6 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

Why negative controls also showing the specific bands of promotor and other regions (GUS, GFP etc.)?

Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...

06 January 2021 8,465 99 View