Pallavi Nigam

5 Questions 7 Answers 0 Followers

Questions related from Pallavi Nigam

How to use a Western Blotting specific antibody for IHC (for PFA fixed & paraffin Embedded tissue)?

I have an antibody in my lab specefic for western blotting only, but I need to detect same antigen in a PFA fixed tissue. I tried to retrieve epitopes using Citrate buffer, HCL treatment &...

10 October 2014 6,859 8 View

Can we use DMSO as a safe solvent to dissolve curcumin for oral administration?

Is DMSO safe for mice since I need to administer curcumin orally to them by dissolving it in DMSO.

05 May 2014 7,184 34 View

how to prepare Ca and Mg free PBS?

how to prepare Ca and Mg free PBS for cell isolation

07 July 2013 5,288 0 View

What can be the reasons for lesser precipitate formation in RNA in situ hybridisation in a PFA fixed & paraffin embedded adult murine tissue?

I am getting very little precipitate in RNA in situ hybridisation for my paraffin embedded adult murine tissue. How can I enhance the purple precipitate in the tissue?

06 June 2013 3,099 6 View

I have 10ng/micro liter DNA template, can it be used for transcription reaction?

Can anyone tell me whether 10ng template sufficient for transcription reaction

06 June 2013 2,155 3 View