I can't purify my bacteriocin from mrs broth culture of lactobacillus gasseri after ammonium sulphate , as its antimicrobial activity get lost?

02 July 2025 0 7K Report

what should be the saturation level of ammonium sulphate so that it's activity remains stable after salting out using cutoff dialysis membrane to proceed further purification steps like - ion exchange chromatography followed by gel filtration.

Badges
Science topic

More Gyan Chand Saini's questions See All

How to conduct the NMR study of a nano-composite film?

Solid state NMR study

03 July 2024 422 1 View

How to find value for Ki (inhibition constant); Kd (dissociation constant), and Km "equilibrium constant?

Hello, I got reviver comment " please look into the comparative assessments regarding Ki (inhibition constant); Kd (dissociation constant), and Km "equilibrium constant" of the target...

09 June 2024 10,069 3 View

Significance off zeta potential ?

Hii experts , i have synthesized a derivativ of biopolmer and zeta potential is a -1.2 mv,,can u tell me how it help me out to study my synthesized product ?

05 June 2024 4,720 3 View

Why Skysat analytic surface reflectance products have wierd reflectance in shadows??

My shadow algorithms did not make sense when applied to the skysat analytic_surface reflectance (SR) image. The shadow signatures were strange i.e. they were supposed to have a strong blue hue,...

27 May 2024 8,090 0 View

Current Trends in IoT Automation?

I am currently delving into the latest trends in IoT automation and am keen to gather insights on the most promising developments in this area. Could you share your experiences or recent findings...

24 April 2024 7,509 0 View

Impact of AI Tools on Academic Research?

As AI technology, such as ChatGPT, continues to advance, I'm curious about its impact on academic research. How do researchers view the integration of AI tools in their work? Are these tools seen...

23 April 2024 7,832 4 View

How to maintain the survivability of endothelial cells?

I am encountering a challenge with culturing endothelial cells derived from umbilical cord tissue. Despite successful isolation, the cells exhibit a decline in viability and begin to deteriorate...

17 April 2024 8,725 3 View

How can we analysed ammonia sensing of a biopolymer ?

I want to know the method of sample preparation in ppm.

08 April 2024 7,242 1 View

Is there a way to add single-item measure in confirmatory factor analysis?

I'm conducting a confirmatory factor analysis and one of my measures has only one item. I'm not able to run the analysis when including that measure, in amos. I haven't even found any research...

30 March 2024 1,577 2 View

Which electrolyte would be suitable for cyclic voltametric study of mild acidic organic compounds?

I used TBAPF6 electrolyte for CV study but it generated anion of my compound. Please suggest me which electrolyte would be suitable for cyclic voltametric study of mild acidic organic compounds?

11 March 2024 1,116 4 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

I want to remove Urea from my protein sample?

I want to remove Urea from my protein (6kDa) sample. I did use : 1. Step down the Urea gradient (dialysis) in order to remove urea and refold my protein, but my protein still contains urea in the...

06 August 2024 1,350 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View