Simultaneous imaging of multiple components (Carbohydrates, Fat, Protein) in food products using confocal microscopy??

Hi Everyone, I am trying to characterize the microstructure (localization of carbohydrates, fats, and proteins) of coconut milk powder using confocal microscopy. From the review of the available...

12 January 2021 5,464 2 View

Is it possible to separate Supleco 37 Fame mix using HP-5MS column?

Hi everyone, I am new to FAME analysis. I am trying to separate Supleco 37 Fame mix using the HP-5MS (30 m * 0.25 mm ID * 0.2 um thickness) column using the following protocol: System: Agilent...

19 December 2020 7,356 4 View

Transcription mediated amplification (TMA)?

I am trying to optimize TMA in my lab. When I start with 150 pg of DNA (Size 235 bp), I get good amplification, which I can visualize as an equivalent size dsDNA product, gel pic attached....

18 November 2020 7,622 3 View

How to do data analysis of a split plot design experiment with an extra control treatment outside the design but in the same field?

In an split plot experiment, 4 methods of Nitrogen(N) application was assigned in main plot and 3 doses of N in subplots and replicated thrice. Along with it, in the same field, No N control...

20 September 2020 9,944 2 View

How do we do intersection of two raster image in ENVI?

After threshold the value of Moran Geary and Getis index using Local statistics in Envi how we get the common part between Moran and Geary indices

05 April 2020 6,234 3 View

How can we extract the Average of Communality from PLS regression results table?

I want to use the value of 'the average of communality' for measuring the goodness of fit indices.

05 June 2018 5,893 3 View

How to reduce noise on xrd data of a metal sample.Is it any software for smooth plotting of graph??

Usually seen that xrd data of any bulk material getting more noise, during tha plot its seems more noisy while completing all basic treatments.

30 March 2018 2,663 3 View

Can anyone suggest me any new method for the root clearing except ink vinegar and KOH, Is it possible that they may not be colonised in the field?

phragmites australis and arbuscular mycorrhiza

12 February 2017 764 5 View

Use of log instead of many digit numbers?

11 December 2014 6,936 4 View

Can anyone assist with PCR amplification of my gene?

11 August 2014 8,932 13 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View

How can I know the stationary phase of growth of several bacteria?

How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...

31 January 2021 4,528 10 View