I am working on the speciation of dermatophytes. I wanted to carry out the Antifungal susceptibility by broth dilution. For standardisation I need Con...

More Sheetal Harakuni's questions See All

How to retain the structure after optimization using Gaussian 16?

Dear Experts I have optimized an organic molecule using DFT/B3lYP/ 6-311G using Gaussian16. However, after the optimization, it was found that some bonds were missing from the molecule's...

17 May 2024 9,828 1 View

How do I take back my paper from preprint on research gate?

I want to remove my article on Quantum key distribution from preprint version on your we bsite.

08 May 2024 2,383 1 View

How to add parameters for "atotypin" ligand type?

I am running docking by using Autodock 4.2 software. However, I am not able to run autodock at the final stage and this type of command error appears in the terminal, Unknown ligand atom type...

10 March 2024 4,317 2 View

Biochar amount calculation with volume ?

I am incorporating biochar treatment in my experiment. I have container volume of 1.5m*1.5.*1m. The application rate of biochar is 20t/ha (i.e 2kg/m2). I will do a surface treatment for 0-20 cm....

23 January 2024 2,473 4 View

Which is the best Web Server for ADMET Predictions helpful for publications ?

I have a few samples and I want to evaluate pharmacokinetics and drug-likeness for the publication, which web server or tool is accepted by the publisher.

13 September 2023 7,489 4 View

Can anyone please help me with DAB enhanced Perl's Prussian blue staining of brain tissue sections?

After staining, the sections appear very light. Should I increase the incubation time in DAB?

18 June 2023 704 1 View

How to assess the purity of mitochondrial fraction obtained by differential centrifugation from mouse brain?

I have tried to check the purity using Abcam's membrane fraction antibody (ab140365). If you have used the same antibody, can you please share your blots?

18 June 2023 743 1 View

Which are the target protien sites in Bacillus subtilis for docking studies ?

PDB Code of protein or name

14 June 2023 1,237 4 View

Which solvent should I use to dissolve NADH?

Can I use Tris buffer (pH 8.0) or deionized water?

13 June 2023 5,536 2 View

How can I obtain good crystals of heterocyclic organic compounds?

Solvent System crystallisation technique

06 June 2023 3,292 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

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How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

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How does the Retention time effect the Biogas Production?

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What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

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22 July 2024 9,576 4 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

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15 July 2024 5,898 0 View

Isolation of Hydrogen Producing Bacteria?

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Hi. Can I get the list of publishers for microbiology-related short communications without publication fee (APC)?

I need to know which journals are exempt from publication fees (APC) in the area of microbiology

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What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

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What are these aggregates in activated sludge flocs?

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Pseudomonas aeruginosa colonies are too transparent: any tips on counting?

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12 June 2024 711 3 View

Karthikeyan Kumarasamy

Hi,

you ask Prof Arunaloke Charabarti, chandigarh ([email protected])

Jay Shah

Tell me what u want to do in detail., coz i have also worked on similar topic..

Umamaheshwari S

Are you working on particular organism?

Sheetal Harakuni

@ S umamaheshwari Mallikarjun I am working on all the clinical isolates. @jay Shah Have you also worked on dermatophytes? Did u use standard strains?

I am working on Candida species isolated from HIV reactive patients.we are speciating and planning for broth dilution assay for drug sensitivity

Actually I am working on Candida with standardisation by standard strains. But finding difficulty in standardising dermatophyte AFT.