Please help me out in how to extract Bacterial DNA by TRIzol even though I am following the protocols properly. If anyone can help me out in extracting good quality bacterial DNA showing 1.7 to 2 absorbance ratio by nano spectrophotometry. My trizol experiment is based on the invitrogen literature but the extracted bacterial DNA is showing less than 1.7 ,i.e. 1.5 or 0.5. Can someone throw me light on this? I am helpless.