The samples are chicken faecal swab, faecal matter, water, sock and tissues.
The media to be used and quantity of samples to be inoculated to get sufficient growth of target bacteria?
Can anybody experienced in this help me please?
Hello, My Sir
my answer about your question , the sample should be taken from tissues of the morbid birds recently or from fecal samples , and the quantity of sample about 1 gram are sufficient to isolate the Salmonella and E.coli .
A larger amount of material might necessary to obtain a representative sample to confirm absence or presence of these microorganisms.
Simple statistics will tell you probabilities for detection at different prevalences of target organism and sample numbers. Single sample size usually is 10-25 g for meat/skin. We used around 10g chicken neck-skin for slaughtered chicken hanging on the chain-line. Search Danish reports for describtion of sock sampling flock status. As I recall this has proven much better than fecal swabs and other single bird sampling.
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