When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

SPSS - Merging files by ID numbers that contain letters?

Hi, I'm trying to merge files from a data base of 20 files, each containing about 4000 subjects. Unfortunately the ID numbers are actually a combination of numbers and letters (e.g., b1458219)....

15 February 2021 5,833 3 View

How would I go about getting an expert review on the interview questions for the dissertation?

Good Evening, My name is David Marshall and I am Doctor of Management Student at Colorado Technical University Online. I just recently received approval from the IRB to start data collection on...

08 February 2021 346 7 View

How can I quantify the color of a solution?

I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...

08 February 2021 2,673 1 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

How does DMSO percentage affect ELISA?

Hello, In my sandwich-type ELISA which is testing for small molecule inhibition of a protein-protein interaction, it seems that DMSO percentage and dilution protocol are important. I...

31 January 2021 6,771 1 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

An interactive strategy for teaching children with autism to focus on specific nonverbal social cues to promote their social skills, of?

An interactive strategy for teaching children with autism to focus on specific nonverbal social cues to promote their social skills

01 February 2021 8,782 3 View

Is there any dataset related to depression detection using social media data?

I am doing my research in depression detection using social media data. can any one suggest me where I can get the dataset for this.

20 January 2021 9,996 2 View

Interpreting regression findings prior to inclusion of control variable?

I'm looking for advice on interpreting a statistical relationship - it's for a paper I am reviewing. (Variable names have been changed). It was found that: [IV anxiety + control variables y and...

06 January 2021 558 3 View

Questionnaires in French and Italian?

Hi to everyone. For my Master's Thesis about ASD in Switzerland I need some questionnaires in French and Italian, as I already have the the German versions. Maybe someone knows if there are...

07 December 2020 3,943 2 View

How to call this type of study please?

Hi there! :) I'm working on creating a follow up study and I need help determining what type of study I'm going to be doing whether its 3x2 or 3x3 please? Details: - I have 2 groups of children...

26 November 2020 4,380 2 View

Why the number of secondary depressions on existing chromosomes varies?

Why the number of secondary depressions on existing chromosomes varies?

15 November 2020 9,614 3 View

No title

As millions are getting depressed losing health and job and loniliness ,anew hope is begining to build up.really we need to this encouragement.we need all hygene ,distant physically to immune...

12 November 2020 159 4 View

What do you think of Buspiron's effect on OCD?

A study performed at the University of Michigan examined whether brain activity is associated with treatment response to exposure-based CBT. Eighty-seven patients with OCD were randomly assigned...

30 October 2020 1,428 3 View

How can we stop mental depression?

27 October 2020 4,429 6 View