I am planning to do Trypan blue staining of leaf tissue, for cell death assay; please suggest any alternative of chloral hydrate for destaining?

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Houda Kawas

Dear Pratyay Seth

Try to find another distributor, better than searching for alternative of the material used

Regards

Prof. Houda Kawas

Eric van der Graaff

It might depend on the plant species and/or organs, but alcohol (70%) also works fine. For Arabidopsis I preferred alcohol over chloral hydrate.

Jean-Christophe Palauqui

try scale A2 for at least 3days (Urea4M in PBS) or a new clearing agent called "Clearsee" (development 2015)

Pratyay Seth

Thank you all, for your kind help. Thanks a lot Eric van der Graaff ; absolute ethanol has worked fine for me.