I am new to HLA typing and related work. Could anyone show me precisely how I can compare my HLA class I alleles with those of other populations?

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I am new to HLA typing and related work. Could anyone show me precisely how I can compare my HLA class I alleles with those of other populations?

I have HLA-A, -B and -C alleles and frequency data and wish to do multiple comparisons

03 August 2016 1,685 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Can I proceed with Response Surface Methodology (RSM) with only one factor for further optimization?

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29 July 2024 5,015 1 View

Recovery Viurses from bacteria genome?

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28 July 2024 8,953 1 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

What role does the gut microbiome play in immune system modulation and disease susceptibility?

Only Probiotics Related

22 July 2024 6,454 0 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

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22 July 2024 9,160 2 View

Use of NC or NTC to descibe negative control?

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18 July 2024 4,183 2 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

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Thomas M.C. Binder Popular answer

Dear Nicholas Nii-Trebi,

Please have a closer look to:

http://www.allelefrequencies.net/

Best regards,

Thomas