04 August 2016 4 2K Report

I have HLA-A, -B and -C alleles and frequency data and wish to do multiple comparisons

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SDS-Gel stacking gel polymerization issues?

Can anyone provide any insight as to why my Tris/Gly SDS-Gels are polymerizing slowly? This especially applies to my stacking gel (image attached) both 4% and 6%. The stacking gel wells polymerize...

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How to use back-trajectory analysis (HYSPLIT) to assess air parcels at breathing/monitor height?

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27 December 2020 730 4 View

Origin of the term "transposition" used in photovoltaic performance modeling?

In PV modeling, the process of elevating GHI data to a tilted surface is often referred to as "transposition". However, strictly speaking, "transposition" in mathematics refers to rearranging a...

22 September 2020 767 3 View

Help figuring out why only lifted cells express eGFP after transduction?

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How to stimulate PBMC-derived monocytes with TLR7 and TLR8 agonists?

I'm trying to stimulate human monocytes from fresh PBMC isolations with 2ug/mL imiquimod and 2ug/mL ssRNA40. I do this in 10% FCS complete RPMI for 18-20 hours before staining and analysing CD14+...

10 August 2020 7,095 1 View

What are the independent, intensive parameters for multicomponent mixtures (and how do I determine them)?

Hi all, For different problems in gasdynamics/thermofluids, different thermodynamic inputs are most convenient for expressing and solving the problem. For example, if I have a stagnation process,...

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What literature should I read to start understanding flow batteries?

I need recomendations on the most core and fundamental materials/literature to start understanting flow batteries from scratch so I can migrate to membraneless flow batteries. Hence, I will also...

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Any recommendations for a high-quality, contract basis, academic illustrator for a paper in Nature Climate Change?

We looking for a high-quality, contract basis, academic illustrator for a paper in Nature Climate Change, any recommendations? Proven track record important.

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