Hello,
I would like to compile a citation reference list covering human RNA-Seq needs for researchers. I am wondering if anyone has already compiled a "guide line" list for the sequencing depth needed for 80% or 90% coverage with RNA Seq for samples of various sources, say FFPE tissue as compared to fresh, or highly expressed sequences as compared to low mRNAs from human or mammals etc.? I realize that it is a big ask but if anyone has some guidance we could work together and share information and the citations..thank you .Sincerely Lyndon
the only way to get such huge citations is write [Human RNA sequence ] in pubmad you will get alot of sequence along with references. 2nd way is to go to expasy server and paste the translated sequence of human Rna you are interested in, search . you will get all possible functional region, snps, mutation etc that are reported upto date along with references.
HI Abid,
Yes I am currently collecting references of articles and making a big list and then I try figure out some guidelines. I was just hoping that active researchers in the field might already have some key references available that I could look at. Everyone today speaks of various depths in generalities but finding true papers for human for specific messages types is more difficult but I am making progress. Thank you for taking time to respond to my questions and I wish you all the best in your research.
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