How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View

Can there be a decrease in the membrane time constant and an increase in membrane excitability?

In my data set, I am seeing exposure to my peptide of interest leads to significant increases in action potential firing responses. But in the same cell types, I am seeing a decrease in the...

17 November 2020 5,441 4 View

What is the best anatomic plane to study BNST neurons using ex vivo slice culture in a rat?

I am looking to study neurons of the bed nucleus of the stria terminalis. I have seen reports using coronal sections. Others present the distinct nuclei in transverse sections. What is the best...

22 October 2020 3,343 1 View

Can anyone recommend a protocol for prolonged storage of mouse brain tissue for eventual paraffin embedding?

My lab is looking to perform IHC staining on the brains of several dozen mice, and we have a need to store the perfused brains for up to a month before we have access to paraffin embedding. Can...

14 October 2020 6,256 4 View

Does anyone have a good protocol for taking soil samples and extracting fungal DNA?

I'm trying to compare fungal communities in the soil of two neighboring ecosystems. I need a protocol for soil sampling to later use for sequencing fungal communities.

25 August 2020 6,830 3 View

Does anyone know what has happened to the International Journal of Advanced Science and Technology (IJAST)?

Dear academics and researchers, Do any of you know what has happened to the International Journal of Advanced Science and Technology (IJAST), since it lost its SCOPUS indexing a few months or so...

05 August 2020 6,949 18 View

What are the independent, intensive parameters for multicomponent mixtures (and how do I determine them)?

Hi all, For different problems in gasdynamics/thermofluids, different thermodynamic inputs are most convenient for expressing and solving the problem. For example, if I have a stagnation process,...

23 June 2020 1,085 2 View

Is there a minimum optimal diameter for neural stimulating electrodes?

With the 1.27mm diameter of DBS electrodes often leading to the development of scar tissue, and evidence that electrodes with diameters near the size of neurons, approximately 30 microns, are less...

26 March 2020 1,782 3 View

Why this orange hue on my gel electrophoresis?

A new batch of 1x TBE buffer was prepared and I was testing it out (result is attached). I am wondering why half the gel is lighting up orange, as I have never seen this before. I have not changed...

12 August 2019 5,209 4 View

Does anyone have any suggetions for a good example of an online 'knowledge bank'?

Hi, does anyone have, or know of, any good examples, of an online 'knowledge bank' - I'm using this as a shorthand for something like a public online repository for their organisation's evidence,...

04 March 2019 202 6 View

Which software is best to plot paleoclimate data with high resolution?

Dear All, I'd like to plot high-resolution figures of paleoclimate data, Please suggest the best and most friendly software. Advance thanks, Saravanan

25 July 2020 3,003 6 View

What programming language can we recommend to a paleoclimatology student?

I will use climatological models in my research work but I have not yet mastered any programming language, now I would like to have advice on the choices to start.

21 June 2020 9,595 9 View

What are some of the free sources where i can download paleo-climate data ? What things you take into considerations while processing paleo data ?

Greetings, I am planning to work with open source paleo-climate data for a thesis, so far the only source for these kind of data i know is : https://www.ncdc.noaa.gov/paleo-search/ Is there...

28 May 2020 9,084 14 View

Can somebody help me with recognizing this fossil plant ?

Please help me in the identification of this well preserved fossil plant found in Tufa . Thank you in advance !

05 November 2019 4,783 6 View

Is there any significance of lacustrine gastropoda fossils for describing paleoenvironment conditions and/or relative dating?

Such as temperature, depth, chemistry, ph. What about for relative dating? Do gastropoda species useful for dating?

08 September 2019 1,464 3 View

What role can engineering play in understanding ancient water technology and water management?

Not only engineering but also Environmental science, Geology, Archaeology, ...

06 July 2019 783 6 View

Does anyone know that what is new topic in environmental archaeology?

Can anyone recommend me a topic for research in the environmental archaeology?

12 June 2019 3,605 4 View

Can any body explain me about significance of TiO2 and S in marine limestone?

I need this answer to interpret paleoenvironment

11 December 2018 1,049 1 View

High resolution AMS 14C dating?

What means high resolution of carbon 14 dating? I.e., If I have one meter sediment core, at 1 cm intervals how many dates should I go to get higher resolution results? Please clarify Looking...

11 December 2018 5,674 0 View

No title

07 August 2018 4,978 3 View