I am doing work with the human pathogen?

More Haleema Sadia's questions See All

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...

20 July 2024 5,405 2 View

Guidance needed for preparing the hydrogel samples for the XRD instrument?

I'm working with a hydrogel sample and I'd like to perform XRD analysis. Can anyone offer guidance on preparing the hydrogel for the XRD instrument? Specifically, I'm unsure about the best method...

20 July 2024 3,611 4 View

How to determine LOD values?

I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...

19 July 2024 1,086 1 View

How to use stress and displacement results from 1st stationary simulation as initial conditions for 2nd eigenfrequency simulation in COMSOL?

Dear All, I am trying to define the dispersion curves of a periodic unit cell in COMSOL Multiphysics with prescribed displacements. I am using two separate studies: - Study 1: Stationary, with...

13 July 2024 7,855 1 View

What is reflexes and legend in XRD analysis ?

The reflexes within the measured XRD pattern are not clearly visible or fully indicated, and a legend is missing. explain this.

27 June 2024 7,257 3 View

How to avoid contamination when applying drugs on your HELA M cells ?

I am passaging my HELA M cells without contamination. After adding cells ( 25% confluency) in 24 well plates I am waiting 24 hours . After that when I am adding my drugs in the wells, after 16...

27 June 2024 2,490 2 View

Is asymmetric term on y-axis in zero field muSR data usually negative?

I am analyzing the zero field muSR data using kubo-Toyabe function. The fit is not converging. I am noted that the asymmetry term on y-axis is neative beyond certain time. I think this is the...

15 June 2024 2,661 0 View

How to upload 16S and metagenomic data from fecal samples to NCBI SRA database?

Hi there, I have some 16S and metagenomic sequencing data from mice fecals and I want to upload them to NCBI Sequence Read Archive (SRA). I don't know what NCBI package is suitable for my animal...

03 June 2024 9,451 3 View

How to find the mass effective density using COMSOL Multiphysics?

Hi everyone, I am attempting to replicate the results of a published paper to determine the mass-effective density in order to elucidate the bandgap formation mechanism. The paper is titled...

16 May 2024 3,272 0 View

How to enrich pig excreta for increasing nutrient quality organically ?

Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.

09 August 2024 5,605 2 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

How is matter moved through an ecosystem and movement of energy different from the movement of nutrients through ecosystems?

03 August 2024 4,859 0 View

Why does the flow of energy through the biosphere depend on the cycling of nutrients and matter and energy flow across the earth's subsystem?

03 August 2024 6,412 0 View

How is the flow of matter through an ecosystem different from the flow of energy matter and nutrients flow through an ecosystem?

03 August 2024 8,655 0 View

From different feedstocks based Biogas digesters what would be the standard NPK - Nutrient contents of the digestate, and how consistent is it?

One has to list down the details and understand that different feedstocks and the biogas digesters working on different feedstocks show different nutrient content value: But when it comes to...

18 July 2024 9,907 12 View

Subculturing of colletotrichum capsici is not able to do please help me?

Hello all, i am working on Chilly anthracnose disease and the pathogen involved is colletotrichum capsici but the problem is occuring while subculturing, i can isolate pathogen from chilly fruit...

17 July 2024 1,610 2 View

What are the key risks associated with foodborne pathogens in imported leafy greens, the impact on public health and effect on regulatory framework?

I am currently writing a systematic review

15 July 2024 9,176 1 View

Many scientists have proven that the use of antibiotics against pathogens in dairy cows has a low success rate. What is the solution ?

I think that in such situations, hygiene measures are very important and the right milking machine is required. Which is the right one to obtain clean and safe milk with a high quality standard?

10 July 2024 4,362 0 View

I'm looking for a tool to evaluate the risk of bias in... Are there any recommended applications or platforms?

Dear researchers, In a systematic review (not a meta-analysis) compiling studies on various diagnostic systems against a specific pathogen. The most relevant parameters evaluated are the type of...

03 July 2024 9,069 3 View

Roger Bayston

Need more information. What is the organism that you are trying to grow?

Haleema Sadia

from wound infection the mannitol salt agar showed good growth but my nutrient agar showed no growth

possibly S aureus with weak or damaged cell wall (can see this in MRSA) so the salt provides osmotic support and prevents the cell from bursting.