Mechanism of DNA replication?

I have read this in a book that DNA polymerase can discriminate between ribo and deoxyribonucleoside triphosphate bcoz of steric exclusion of rNTPs from the DNA polymerase active site, as the...

08 June 2020 2,262 6 View

I want to carry out analytical method development method for gadobutrol drug a MRI contrast reagent . Not able to get uv in these .could anyone help??

I not able to carry out uv response on these could any one suggest how to take uv as these compound dont have any chromophoric group present in these .

09 February 2020 3,291 3 View

Low library yield using Illumina True seq small RNA library prep ?

I am looking for suggestions to improve the yield of my libraries prepared using Illumina True seq small RNA library prep kit. Input RNA is purified small RNA (50 ng) and I run for 15 PCR cycles....

03 February 2020 2,645 2 View

Why my single stranded RNA bands don't appear on a denaturing agarose gel?

I am trying to check the size of my single stranded RNA on agarose gel with a denaturing loading dye in TAE buffer. Although ssRNA ladder bands are appearing but my samples (having 25-100 base RNA...

10 April 2019 4,091 3 View

Can someone help me with Real time PCR of genes at acidic pH?

Hi All, i am working real time PCR of certain genes at acidic pH. i have done the same experiment 4-5 times one year back and i got upregulation of certain genes. This work is also published by...

31 January 2016 3,721 4 View

Which is optimum cDNA concentration for RT PCR run?

Please suggest the optimum concentration of cDNA for RT PCR run. Does diluting the cDNA 1:10 or 1:100 help in increasing amplification, specially for low expressing target genes?

06 January 2016 6,148 4 View

Why can I get no ct value for my RT PCR experiment?

I am trying to amplify cytokine genes in cDNA derived from macrophages but unable to get any amplification using comparative delta Ct experiment. Cross checked mRNA concentration and purity which...

05 January 2016 9,725 5 View

Is Ct values above 35 in RT PCR run acceptable or not?

Can anyone suggest me whether Ct values above 35 in RT PCR run is acceptable or not? And whether it signifies primer dimer/ non-specific amplification. What is the acceptable range of CT values in...

05 January 2016 6,830 27 View

How to remove protein contamination from RNA samples?

27 July 2015 7,807 14 View

How can I increase the solubility of a compound for an MTT assay?

The compound used for MTT is not soluble in water and sparingly soluble in 5% DMSO, due to which there is issue with uptake in the cells. How can this be solved?

08 July 2015 8,571 14 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View