Hw specific is the estimation of the RNA concentration estimation using the orcinol method ?

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Greg Cote

Typically, orcinol is used for quantitation of carbohydrates, with pentoses and hexoses giving different colors. So, if you are trying to measure RNA in the presence of other pentose-containing carbohydrates, I would say it's probably not very specific.

Adam Hannon-Hatfield

Why not use A260?

Ayodeji O. Olarinmoye

A modified orcinol test for the specific determination of RNA has been described (Almog and Shirley, 1978). The essential differences between this method and other methods are as follows:

Preincubation of the samples with H2SO4 before addition of the orcinol reagent;

Decreased concentration of orcinol;

No addition of FeCl3.6H2O;

and quantitation of RNA at its maximum absorbance at 500 nm, where interferences from DNA and proteins are minimal.

Reference

Almog, R., & Shirey, T. L. (1978). A modified orcinol test for the specific determination of RNA. Analytical biochemistry, 91(1), 130-137.