what is the difference between UV/DAD detector and UV/VIS detector
The DAD detector can scan the UV and Visible spectrums but also perform peak purity analysis. Thus, a DAD is used more in method development and validation (specificity) in order to demonstrate that no impurity co-elutes with the molecule of interest (related substance).
A UV/Vis detector can determine the absorbance at a particular wavelength (whether fixed or multiple wavelengths) but also 'blast' more energy through the sample. However, it cannot perform peak purity analysis.
A DAD can be used for routine QC analysis, but it is a waste of resources.
Kindly check here https://www.allinterview.com/showanswers/.../what-is-difference-of-uv-and-pda.html
Dear Krishanthi,
You may also like to know that there are ultra-fast scanning UV/Visible HPLC detectors, which can offer the advantages of a photodiode array detector. Some have ultra-low drift and noise. Hence they may be used for providing detailed spectral information of eluting chromatographic peaks and for quantitative measurements of eluted peaks, within the same chromatographic elution.
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Ade Kujore
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what is principle of the HPLC-UV/DAD analysis thanks
Dear Achille,
Please see this page for a general summary. http://www.cecilinstruments.com/introduction-to-hplc-and-ion-chromatography.html
In the case of HPLC-UV/DAD analysis, analytes or derivatives of the analytes, are detected by virtue of their ability to absorb light at UV/Visible wavelengths. Various types of UV/Visible HPLC detectors, which Bruce and I have outlined, may be used.
Ultra-fast scanning UV/Visible HPLC detectors, can also determine chromatographic peak purity.
Kind regards,
Ade Kujore
>
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Cecil Instruments Limited
Milton Technical Centre, Cambridge CB24 6AZ
United Kingdom
>
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Reduced noise in UV, and more detailed. DAD is capable of purity configuration of peaks, good for validation of methods.
This is an example of a question asked many times before. Please try a search first, before posting. Here is an example of the same question being asked, with responses. https://www.researchgate.net/post/DAD-vs_UV-detector?view=55c2670c5dbbbdc7ac8b45be
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