How would one qualify and quantify lipid within a mix of water, alcohol, surfactants, and biomass?
first I think you have to centrifuge your samples and you will have then you will take the supernant which contain lipids which will be dissolve in ethanol and water , then make liquid liquid extraction you will have your and you will have two layer the upper layer will be for ethanl with lipid and the lower layer will be for water which have high denisty you take the upper layer and if you still have water you can remove it from your sample by adding spoon of Na2SO4 anhydrous , and later you make evaporation for ethanol and you will have lipid oj the wall of your flask after evaporation then you can start to prepare it for measuring with GC/MS analysis
Thank you for your answer, Mohammed. Could it not be possible for centrifugation to force the oil out? Some algae cells are that fragile. I deal with Chlorella and Nanochloropsis both of which are rugged but I am not sure of how long I would have to centrifuge without having to worry about whether or not cell disruption was brought about by centrifugation.
you dont need long time to centrifuge I think , that is only to spearte the biomass
I would recommended 3 or 5 mintues at 5000 rpms,
may be you shall try it after 1 , 2 , 3, 4, 5 mintues and then if you obtained th seperation after 1 mintues you can continue it that way.
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