Calculating the CFU?

i am constructing research for the antibacterial activity of the medication and I am testing it by %RCC, so my Q after I did 10 serial dilutions for the bacterial suspension can we do plating for...

04 February 2021 5,886 1 View

How to determine the types of gene resistance of beta-lactamase to perform to my samples by PCR?

Greet to everyone. I did antibiotic resistance by vitek2 compact and I found beta-lactamase resistance phenotypically in Gram-negative bacteria. How can I determine types of bet-lactamase genes to...

05 September 2020 8,898 5 View

Hi, I want to run simulation with YASARA for different part of my protein separately but I dont know how to run?

I am working on enzyme engineering and want to run the simulation for my enzyme the open and closed conformation to see the effect of the mutation on my enzyme as its located on the lid. However,...

10 May 2020 2,625 1 View

Are there any guidelines to choose antibiotic genes resistance for beta-lactam to do multiplex PCR?

I isolated some Gram-negative isolates that appear high resistance to most antibiotics. I want to do multiplex PCR to isolates for beta-lactam gene resistance. are there any recommendations to...

22 February 2020 4,842 6 View

Is there a way to transform a function of two variables into one variable?

Hello, Assume that I have Z=f(x)+g(y). Is there a way that I can write Z as a function of one variable w that relates f(w) and g(w)? Thanks in advance for your help,

29 August 2019 3,132 1 View

Does boiling method for extraction of bacterial DNA is suitable to obtain bacterial and plassmid DNA to work on antibiotic resistance genes?

I work on antibiotic resistance genes in bacteria. Resistance genes present on plasmid. I extract DNA using boiling method. Does this method suitable to obtain plasmid DNA to study resistance...

04 April 2019 6,639 15 View

Sample size in experimental probability?

according to the probability theory, suppose that we calculate the experimental probability of students who prefer mathematics and it was .70%from a sample of 20 students (14/20), is that correct...

06 January 2019 3,681 6 View

Is international journal of innovative research in information security a recognized journal? or it is a fake one?

I want to publish a paper at a journal but I am confused where my experience is not too good to differentiate between fake journals or not.

22 December 2018 4,472 2 View

What is the best taxon sampling that lead to draw a reliable conclusion about the floristic affinities of entire flora?

I'm interested in studying the phylogenetic affinities of the flora of arid, nearshore archipelago (less than 100 km from nearest mainland). 300 species fall in 129 genera were recorded, no...

18 September 2016 499 3 View

Which one is the best, bulk polymerization or precipitation polymerization?

14 April 2016 732 4 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View