Steps to improve cryoEM helical reconstruction resolution using relion?

Hello all, I am trying to do helical reconstruction for my fibrils. I am at 3D classification steps where I can get the resolution (given by relion) at 3.2 A. The actual resolution is worse I...

28 July 2020 6,266 1 View

Does Aristotle have a term and a theory for what the Scholastics have named "ens rationis" as opposed to "ens reale"?

Scholastics have called "entia rationis" those objects, which do not and cannot exist in reality but only within the mind, such as chimaeras, privations (like blindness), negations...

19 July 2020 4,667 22 View

Are there contemporary evaluations of US public health system?

I'm looking for a recent evaluation of the US federal efforts to prevent disease outbreaks, specific to the US public health system. I see some old (15-16 yr old) reports, anyone done anything...

06 June 2020 7,396 1 View

Is there any working method for concentration of influenza virus from water and sediments without the inactivation of the virus?

I have done an experiment to study the persistence of avian influenza in lake water and in lake sediments. Do you know a good concentration method to use without the inactivation of the virus from...

27 January 2020 7,044 3 View

There is any way to know the equivalence between an old genome reference assembly and its new version??

28 November 2019 8,768 4 View

Peptide concentration for stimulating murine CD4+ and CD8+ splenocytes?

I'm trying to figure out a good starting concentration of 15mer peptides to stimulate mouse CD4+ and CD8+ splenocytes for cytokine staining. We are pooling 72 peptides for one of our conditions to...

17 October 2019 6,974 2 View

Can commercial Competitive ELISA Oxytocin kits measure synthetic oxytocin (used in clinical practice)?

Can commercial Competitive ELISA Oxytocin kits measure/pick up synthetic oxytocin (used in clinical practice)?

09 September 2019 6,479 3 View

Is there a statistical techniques to compare multiple subscores of a specific domain?

I performed a neuro-psychological battery consisting of 6 tests that measures different aspects of executive function. Each test has between 3 to 15 subscores. There is no consensus on whether one...

10 June 2019 4,062 3 View

How long does an IAC antibody-coupled NHS column last?

I'm trying to use IAC to purify a protein using an old prepacked NHS column with a potent antibody, that was coupled 2-3 years ago, and getting bad yields (Wide peak over 4 mL at 2 mAU max,...

17 January 2019 5,664 4 View

Use examples of mathematical minimal surfaces?

I am interested in the practical uses of mathematical minimal surfaces in engineering design (such as gyroids, Schwarz surfaces, etc.). Can anyone provide good examples, particularly in art and...

07 October 2018 5,011 10 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

How can we add reinforced thermoplastics with granules in injection molding machine?

I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...

22 February 2021 4,290 1 View

Why is my qPCR suddently not working?

I've been working with qPCR for some months now. I amplify 2 genes (PSEN2 and HN1), and the protocols are optimized for both and have been working fine. The other day I amplified HN1 for some...

21 February 2021 3,437 3 View

Drop weight impact testing machine for composites?

Dear all, I hope everyone is safe and healthy I do have a question please. Where can I find a drop-weight impact testing machine to test fiber reinforced polymer composite plates (CFRP and GFRP)...

17 February 2021 3,820 3 View

Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

What kind of tape can be used to fix the samples on an XRD machine?

I have a very thin sample, the thickness is around 1 mm. I want to do X-ray diffraction measurement on the thin side of the sample. What kind of tape can I use to fix the sample, and will not...

13 February 2021 4,153 3 View