How to visualize biotin on TLC plate?

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Adam Zmysłowski

Ninhydrin mostly reacts with primary amino moiety and biotin has secondary amino. Maybe increasing the concentration and the temperature would help but I would such change to something like 4-dimethylaminocinnamaldehyde

Article TLC Determination of Biotin in a Lyophilized Multivitamin Preparation

Bongo Mount-Batten Majeke

You could try a 0.4% methanol solution of 2,6-dichloroquinone-4-chloroimide.