Hi dear colleagues's, I'm looking for a protocol to how use protease inhibitor in antibacterial effect measuring of mucos and serum of fish ? and want to know which protease inhibitor Drug i can use for fish skin mucos?
Hi Hojat, I used to work a lot with fish tissue and I don't know of any proteinase inhibitors that can inhibit bacteria.
High salt, low pH and low temperature are much more practical to inhibit bacterial growth in fish tissues.
Plus, simple agar plates or fish samples spiked growth medium is enough to monitor bacterial growth in fish tissues.
I think a protease inhibitor should be used when the antimicrobial activity is tested against bacterial strains producing of proteolytic enzymes that can hydrolyze the glycoproteins of fish mucos.
Thank's for answer Dr. Ferdes, the reason of using protease inhibitor is blocking proteolytic enzymes pathway, but i want to know how i undrestand i blocked whole of this pathway successfully.
Hi Hojat, major bacterial proteinases are either metallo-proteinases, subtilisin-like serine proteinases or thiol proteinases.
The former can be blocked by EDTA, bacterial serine proteinases can be blocked by PMSF or diisopropylfluorophosphate (DFP) or their derivatives and the thiol proteinases can be blocked by iodoacetamide or maleimide..
You can block all these bacterial proteinases, but, it will result in a chemically laden fish mucosa that is unfit for human consumption because of all these toxic inhibitors
Thank's u Mr Stefansson for ur useful answer, do u have any opinion for inhibiting cathepsin protease (cathepsin B & L) and aspartic protease (cathepsin D)?
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