We are going to use the Heidelberg OCT Spectralis for mouse OCT acquisition. However, we find several papers reporting the use of this instrument for rodents, we are not able to successfully do it. We have two separate devices (1- Heidelberg OCT Spectralis and 2- Heidelberg Hra 2). The Heidelberg OCT Spectralis has two lenses one 30° Standard Objective Lens and an Anterior Segment lens. The Heidelberg Hra 2 has 55° widefield lens. unfortunately, the 55° lens could not be assembled to Heidelberg OCT Spectralis.
As far as we know, given the high dioptre of the mouse eye, we should use the 55° widefield lens. However, using the standard 30° we get a rather acceptable cSLO image, no OCT image is displayed.
Can anyone help solve this problem? We already tried using an additional lens in front of the device lens but still not working, however, maybe the total dioptre of the lens was not enough.
Also, a paper suggests minor software modifications (using Alt+Ctrl+Shift+O in Heidelberg Eye Explorer software) which we could not figure out how that should be done. (Spectral domain optical coherence tomography in mouse models of retinal degeneration. Invest Ophthalmol Vis Sci. 2009 Dec;50(12):5888-95. doi: 10.1167/iovs.09-3724.)
These are some papers about using the Heidelberg OCT Spectralis for rodents:
1- Quantitative Analysis of Mouse Retinal Layers Using Automated Segmentation of Spectral Domain Optical Coherence Tomography Images. Trans. Vis. Sci. Tech. 2015;4(4):9. doi: https://doi.org/10.1167/tvst.4.4.9.
2- Tracking Longitudinal Retinal Changes in Experimental Ocular Hypertension Using the cSLO and Spectral Domain-OCT. Invest. Ophthalmol. Vis. Sci. 2010;51(12):6504-6513. doi: https://doi.org/10.1167/iovs.10-5551.
3- Giannakaki-Zimmermann H, Kokona D, Wolf S, Ebneter A, Zinkernagel MS. Optical Coherence Tomography Angiography in Mice: Comparison with Confocal Scanning Laser Microscopy and Fluorescein Angiography. Transl Vis Sci Technol. 2016 Aug 18;5(4):11. doi: 10.1167/tvst.5.4.11. PMID: 27570710; PMCID: PMC4997887.
Vahid Mansouri
Take a look:
1. https://www.youtube.com/watch?v=AINF5rK48RY&t=18s
2. https://www.youtube.com/watch?v=Rx5R6acoxNw
you can see the following links:
1. https://www.heidelbergengineering.com/media/e-learning/Totara-US/files/pdf-tutorials/2238-003_Spectralis-Training-Guide.pdf
2. http://stmichaelshospitalresearch.ca/wp-content/uploads/2015/09/Spectralis-Quick-Guide.pdf
3. https://www.heidelbergengineering.com/media/e-learning/Totara/Dateien/pdf-tutorials/99913-005_Spectralis_HRA+OCT_Acquisition_Brochure.pdf
Qamar Ul Islam
Thank you for sharing the videos. However, I do not get my answer yet. Do you look at the papers I mentioned in the question?
Vahid Mansouri I've come across the same question and I was curious if you were able to find a way to use the Spectralis for mice imaging?
Danilo Andrade De Jesus
Hi Danilo. Unfortunately, no. We could only get some preliminary results using TOPCON using 7line raster mode. however, we could not achieve a full field of the retina.
- Badges
- Science topic
- Similar topics
- Chemistry
- Pharmacology
- Pharmaceuticals
- Drugs