How to trypsinize over-confluent SiHa cell line?

07 August 2019 0 2K Report

When I revived SiHa cell lines, they were over-confluent just in 24 hours. When I tried to trypsinize with 0.25% trypsin (with 0.001% EDTA), cells were not detached .

How to solve this problem?

Badges
Science topic

Similar topics
Comment

More Sudip Panday's questions See All

How to do lncRNA annotation in R after making count matrix?

Can anyone please

30 April 2024 1,444 0 View

What is the purpose of fixing Macrophage 264.7 & Dendritic cells (DC2.4) for the surface marker expression with flow cytometry? What if I don't fix?

I already did some activation experiment to observe expression of CD40, CD86, CD80 and MHC with macrophage 264.7 and dendritic cells (DC2.4). I treat these cells with positive control LPS and...

05 April 2024 4,628 1 View

What does panel design mean in flow cytometry? Can someone please help me?

Previously I did some surface marker expression (CD40, CD86) of RAW macrophages and DC2.4 cells using flow cytometry. For RAW cells, results were not satisfactory. Now, I am optimizing apoptosis...

30 March 2024 7,535 2 View

How can genomic selection be used to improve the efficiency of plant breeding programs?

07 July 2023 5,053 2 View

How can permaculture contribute to soil regeneration?

01 July 2023 5,898 2 View

How does permaculture address energy efficiency?

01 July 2023 5,534 1 View

What is the role of animals in permaculture systems?

01 July 2023 9,432 1 View

How can permaculture be applied in urban settings?

01 July 2023 7,986 1 View

What are the challenges of implementing permaculture on a large scale?

01 July 2023 3,050 4 View

How can we remove harmful compounds from mustard oil?

How to remove harmful compounds from mustard oil?

13 June 2023 8,128 0 View

How to draw the five chelate rings of [Co(EDTA)]- on this picture?

Is this circle one of the rings?

03 August 2024 5,988 0 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...

28 July 2024 8,027 3 View

How can EDTA be removed from already extracted DNA samples?

Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...

13 July 2024 1,675 3 View

What could be plastic adherent cells from PBMCs maintained in High Glucose DMEM 15% FBS ?

I'm trying to obtain MSCs from whole blood PBMCs and from the CD34- fraction of umbilical cord blood. However, I'm obtaining other types of cells that prevent the MSCs from growing. These cells...

26 June 2024 3,664 1 View

Any tips for cell counting in 96-well plate?

Dear colleagues, I am currently using HCT-116 adherent cells for cell culture in 96-well plates and have some questions regarding cell counting. I have tried various methods and found the most...

22 June 2024 1,124 5 View

3T3-F442A cells not adhering to coverslip?

Hi, I am attempting to culture 3T3-F442A cells and carry out IF staining. I'm testing a variety of different compounds on the cells (e.g. lopinavir, clozapine, olanzapine etc.) Up until recently,...

16 June 2024 2,754 0 View

For nuclei extraction from human cells, can I replace EGTA with EDTA in lysis buffer?

I understand that there are differences in ion preference as far as chelation is concerned when comparing EGTA vs EDTA. But is it ok to replace EGTA in my lysis buffer with EDTA since EDTA...

10 June 2024 1,188 1 View

Fibroblast trypsinization in Hanks medium with Ca and Mg?

We are setting up fibroblast cultures in a new location and we had to change the mediums suppliers. At the trypsinization step trypsin did not work and we realized our hanks medium has Ca and Mg....

28 May 2024 6,052 2 View