Why my lenticrisprv2 plasmid after ligation of oligos is of smaller size than the expected?

after miniprep, I sequenced the plasmid. After sequencing I found that a region of plasmid was missing and its size if of around 8kb while it should be of around 12kb. It has happened to me for...

21 March 2020 736 3 View

What statistical parameters could be used for estimating "altitudinal variation in orthoptera species richness and diversity"?

23 March 2019 1,144 2 View

Appropriate methodology for species documentation of odonata?

I am starting a research on odonata diversity of Agriculture and Forestry University, Rampur, Chitwan, Nepal. I will be taking samples/specimens (only adults) on a weekly basis from the wetland of...

07 March 2019 5,705 5 View

Requirements for Images(dataset) for an image classification problem?

what are the ideal requiremnets for data which should be kept in mind when data is collected/ extracted for Image classification. What are different techniques in machine learning or deep learning...

21 November 2018 8,694 5 View

Recently many research has been carried out at the nano-bio interface. What are the best way to deal with nanomaterials toxicity issues?

Nanotechnology has made tremendous growth in every domains of science and technology. However, nanotoxicity has been become the serious issue. How to minimize the toxicity of nanomaterials in...

10 September 2018 2,133 4 View

Measurement of flow of water in millimeters with a suitable sensor which will be integrated in a Wireless Senor Network?

Hey, How to measure the flow of water in millimeters and for that case, I am looking for a suitable sensor which will be integrated into a Wireless Sensor Network. I have limitations on power...

11 June 2018 9,944 3 View

Is anybody working on Financial Inclusion in India?

09 April 2017 1,266 5 View

How to interpolate with 2 variables in R or ArcGIS?

28 July 2016 4,599 4 View

Is it necessary to add time dummies in random effect model?

In fixed effect model we usually add time dummies, is it necessary to add time dummies in random effect model also?

07 July 2016 5,231 4 View

How does one select spore forming anaerobic bacteria by heat treatment?

I would like to select spore forming anaerobic bacteria by heat treatment. Can anyone tell me how long and at which temperature i shall heat liquid culture to select spore forming bacteria and...

09 September 2014 5,631 6 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View