Dear researchers,

i want to test for statistical differences between different sample sites. I´ve sequenced microbiome of 16 biogas plants and 10 sewage treatment plants, and get an otu table, which comprise OTU´s absolute abundancies for each plant.

Now i would like to test whether the OTU composition is significantly different:

A) between the two groups of plants

B) between each plant

For A i would do ANOSIM and for B i would prefer an one way Anova.

Is that acceptable or are there any other suggestions?

Should i do the analysis with the relative or absolute abundance. Is it necessary to transform data before analysis?

Thanks for your help, as you might already know, i´m no statistic crack.

Thank you very much,

Christian