I am trying SNP detection with the High Resolution Melting method (with Viia7), but have to face the problem that my DNA isolates are heterogeneous in DNA and buffer concentrations.

It is impossible to change the isolates, so I wonder what else could be changed to increase the performance of this method?

I have an idea to use inner temperature controls, but that requires temperature-shifting. If I understand correctly, this tool isn't included in Viia7 software. Any suggestions?