Alexa Fluor 488 dyes are disulfonated analogs of Carboxyrhodamine 110. I have a protocol from a patent by Haugland. Has anyone tried synthesising this via some other method?
Pratik
This chemistry work fine, almost as described in patent you were referring, but workup and purification is not straightforward, but works well according to this patent. Making NHS ester and its purification is more troublesome compared to making Alexa Fluor 488, it just hydrolyzes much faster compared to other NHS esters I worked with. Nowadays you can buy Alexa Flour 488 NHS ester and most of other Alexa Flours (kind of generic Alexa Fluors) at very reasonable price. Here is a link to one supplier.
Alexa Flour 488 NHS ester https://fluoroprobes.com/product/af-488-nhs-ester/
Alexa Flour 594 NHS Ester https://fluoroprobes.com/product/alexa-fluor-594-nhs-ester/
Many other Alexa Dyes are available from
https://fluoroprobes.com/
I had the same problem. The reaction worked but the purification was a big pain. Thanks for sharing your experience.
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