Pratik Kumar

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Questions related from Pratik Kumar

How do you get rid of TFA from HPLC purified sample?

I have purified a small organic molecule using 0.1% TFA/Acetonitrile and 0.1% TFA/Water. Now due to the presence of TFA in my solution, I am not able to obtain proper mass using ESI-MS (due to low...

01 May 2014 2,899 7 View

How to synthesize Alexa Fluor 488 dye using Carboxyrhodamine 110?

Alexa Fluor 488 dyes are disulfonated analogs of Carboxyrhodamine 110. I have a protocol from a patent by Haugland. Has anyone tried synthesising this via some other method?

24 April 2014 3,240 2 View

How do I form an ester using pentadflurophenol?

This organic acid also has a relatively non nuclephilic hydroxyl group. I need to activate a carboxylic acid.

17 April 2014 1,490 16 View