Hi. If I were to supplement a 500ml media with two different antibiotics of the same concentration (10μg), how do I make sure the concentration of each antibiotic doesn't change?
Apparently, my strain (which is ampicillin-resistant and tetracycline susceptible) grows on BHI agar supplemented with 10μg ampicillin and does not grow on BHI agar supplemented with 10μg tetracycline, which is fine. However the bacteria grows on BHIA supplemented with tet + amp, both 10μg. Could this possibly be caused by any change in the antibiotics concentration?
If the antibiotics are in powders, make it into an equal percentage solution. And try again.
No, it would not be due to any change in the antibiotic concentration, they are independent of each other (assuming that the plates were made correctly). You should look to some other explanation.
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