I am trying to make PLGA-PEG nanoparticles. For this, first I make PLGA-NHS from PLGA-COOH bu using PLGA-COOH , NHS and EDC in DCM.
I precipitate it (PLGA-NHS) using Methanol.
Further, I use this PLGA-NHS with PEG-COOH + DIEA in Chloroform.
The reaction is kept on stirring for 24hr. But when I precipitate it, i don't get a proper yield. The maximum I could get is 3% yield, which is unacceptable.
Can anyone tell me which can be the problems, or critical steps .
Thanks.
have you tried to precipitate it with Petroleum ether?
I have done that before but not from PLGA-NHS from another material ( PLC).
Hi, Thanks Bunian.
I figured it out. Concentration was the main issue. Increasing the concentration gave me the product.
Hi Tanzeel, this is a while after your experiment, but would you be able to give the concentration of PLGA and PEG you used for your synthesis? I'm running into a similar problem myself so it'd be very helpful to hear what ratios/concentrations you used. Thanks!
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