If you look for cytokins in blood or transcription factors in cells, you might have some problem see these proteins with 2DE.

The composition of blood is well known, so you could check in proteomics papers if the authors have already identified your proteins.

Thanks, stéphanie

Dear stéphanie, thank you for your reply, but if you want any explanation for that, any references talking about your opinion, i would be grateful, Regards!