Is the method of storage anaerobic bacteria at -80 in Glycerol stock same as that of aerobic bacteria? Will Glycerol prepared in degassed water be able to save bacteria?
Using glycerol the same way you do for the storage of your aerobic organisms is sufficient. If you are able to, I would suggest you use/try out the Protect Beads which can be available from Thermofischer. In the end of the procedure for storage the excess glycerol is removed from the tube so that bacteria are stored in the beads. As you need to sub out the organism you remove a bead and let this thaw out onto your plate before streaking out. Here you do not have to worry if the concentration of glycerol is right or not.
I have attached a document on this for your perusal.
yes!! you can store anaerobic bacteria at -80C in degased glycerol
We use 50% Brucella medium and 50% Glycerol (40%). So the Glycerol has a final concentration of 20%
Using glycerol the same way you do for the storage of your aerobic organisms is sufficient. If you are able to, I would suggest you use/try out the Protect Beads which can be available from Thermofischer. In the end of the procedure for storage the excess glycerol is removed from the tube so that bacteria are stored in the beads. As you need to sub out the organism you remove a bead and let this thaw out onto your plate before streaking out. Here you do not have to worry if the concentration of glycerol is right or not.
I have attached a document on this for your perusal.
Yes.I make glycerol stock for aerobic and anaerobic bacteria in the same way.
Thanks all for answering the question. Hope soon our bacteria will be preserved.
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