I am using Perkin Elmer Clarus 480 GC instrument. Can anybody tell me how to standardize it? Whether we should use any internal or external standard and how to do it?
For which types of compounds that you want to be standardized the GC instrument. eg. essential oils or any other applications. In case of essential oils or aroma flavour compounds you can use the GC in split mode and for low or minor volatile compounds use splitless mode. For quantification of each volatile compound you have to use run the internal standard on same conditions. For all these follow good published methods on GC or GC-MS (Flavour and fragrance journal, Food chemistry, J Agr & Food Chemistry etc.). Then look your chromatogram and play with ramping. You will get your desired results.
Gud luck
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It may be important to use an alkane mix as standard to be able to calculate retention indices for the compounds in your samples. Serveral journals meanwhile insist on such indices as additional tools for compound identification.
Best regards
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As I understand you want to calibrate the performance of GC instrument.
If yes, then kindly note that each detector has particular compound with specific concentration which is recommended by manufacturer. The basic test for calibration of instrument are sensitivity and reproducibility which should be perform according to the protocols provided by the manufacturer. these information you can get from authorized dealer or from web site of that manufacturer.
You can find standards for the particular column you are using. Standardization related to particular column and you can also check on the internet your column.
The GC instrument is calibrated by using known stds . Five times same micro liters volume are injected in injector port of GC having same GC condition and using same capillary column. We have to find out Std deviation and % RSD. This is one way to standardize GC.
For pesticide GC instrument is calibrated by using known standards . Six times same micro liters volume are injected in injector port of GC having same GC condition and using same capillary column. in this way we can get standard deviation of the instument. One thing you should take care in autosampler that vials containg solvent (Hexane:Acetone) should be change after injection in GC.
I am not sure what do you mean by standardization of GC. I think you mean to say how to evaluate the Performance of a GC. If thats the case, you need to verify the performance of detector, injector, oven and autosampler. Generally an alkane mixyute is used to do this. We use n-octanol in hexane for this purpose. Linearity, reproducibility in area and retention time, carry over tests etc. are carried out for split as well as splitless injections. You can check the website of OMCL Network of the Council of Europe. As far as use of the internal or external standard is concerned, it depends on the analyte and purpose of the analysis.
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